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If the manufacturer of a medication specifies a particular diluent (e.g., sterile saline), bacteriostatic water should not be substituted unless approved, and if used, the osmolarity of the mixture must be considered

0.8% bioavailability

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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six