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Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

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As for post-care, youll want to avoid pressing and manipulating the treatment area for 36 hours, to allow filler to naturally settle into its new home

Lenzi A, Picardo M, Gandini L, et al