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For years, this was one of the hardest questions in the field, because receptor localization was confounded by nonspecific antibodies that produced false signals in tissues that turned out not to express the receptor at all

Gentle swirling minimises this interface disruption, which is why reconstitution protocols universally recommend rolling the vial between your palms rather than shaking

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A daily folic acid supplement of 400 mcg, or methylfolate 500 mcg if you have MTHFR gene variants, maintains adequate levels