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If digestive upset persists despite these strategies, discontinue use and seek professional guidance

These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

These may be symptoms of gallbladder problems (eg, cholelithiasis, cholecystitis)

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To assess whether inflammatory changes are pro- or anti-inflammatory we also assessed cytokines related to TH1 and TH2 cells