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Cell lysis and protein extraction For protein extraction, cells were disrupted with a cell scraper in a lysis buffer consisting of 50 mM HEPES-Na, pH 7.2, 0.5 M NaCl, 0.5% (v/v) NP-40, 0.05% LDS and 1Pierce EDTA-free protease inhibitor cocktail (Thermo, Australia) in Milli-Q H 2 O and then vortexed

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Glutamate can also be deaminated via glutamate dehydrogenase to regenerate -ketoglutarate and release ammonia, a mechanism well-document in vertebrates for nitrogen and anaplerotic balance 74

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The peptide stability guide compares shelf life and degradation profiles across commonly used peptides

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