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Erythrocytes were lysed twice by adding DEPC-treated H 2 O and NaCl (8.8%), followed by centrifugation (450 x g, 6 min, 4C, Universal 320 R, Hettich, Tuttlingen, Germany)

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Indeed, others have previously reported that N-OH amphetamine produces amphetamine-like behavioral effects and that, in some assays, it is at least as potent as amphetamine [2,18]