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Disulfide conjugation protocol The pure thiol-containing peptide and thiopyridyl-activated linker-functionalized MK-801 were dissolved in DMF (2 ml) and buffer consisting of 6 M guanidine and 1.5 M imidazole in H 2 O at pH 8 (200 l) and agitated for at least 2 h

371-OR: Reduced hepatic expression of the mitochondrial quality control factor PARKIN hastens the progression of NAFLD in mice and is associated with NASH in patients

Epub 2000/03/29

extended storage is moved to 20 C aliquots [10]

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LDH release was assayed by ELISA in duplicates from aliquots of cell culture media