Adhesion to HT29 cell lines The HT29 human intestinal epithelial cell line was routinely maintained in High Glucose DMEM (Dulbeccos Modified Eagle Medium) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 50 g/mL L-glutamine, and 40 g/mL gentamicin at 37C in a 5% CO atmosphere (all reagents from Euroclone
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The duration of treatment was 3 days