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Ongoing Care: This is not a set it and forget it program

Yes, lyophilized (freeze-dried) vials do best refrigerated at 3646F (28C), tolerate cool room temperature for short periods, and last longest frozen at -20C (-4F) or colder

This study was supported by Project of Baima Future Foods Research Institute Undertaken by Best Candidates Selected through Open Competition Mechanism (grant number: JBGS-2021-004), Project of Jiangsu Province older adults health research (grant number: LKM2023050), the Natural Science Foundation of the Higher Education Institutions of Jiangsu (grant no: 24KJB350010), and Youth Science and Technology Project of Suzhou Vocational Health College (szwzy202324)

Consequently, HILIC method development and validation require thorough risk assessment during method development to identify and minimize sources of variability and ensure robust methods suitable for GMP settings (which is entirely possible!)

(5) Berger DR, Centeno CJ, Steinmetz NJ