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Antibiotics and adverse events: the role of antimicrobial stewardship programs in doing no harm

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The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C

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MT-2 (Melanotan II) is a synthetic peptide supplied as a high-purity lyophilised powder (HPLC/MS verified)