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The suspended liver microsomes were placed on ice in prechilled microcentrifuge tubes, allowing for the addition of 640 ul of 0.1 M Tris buffer and 10 ul of 500 uM test compound to the sample
Gill EL, Raman S, Yost RA, Garrett TJ, Vedam-Mai V
Sci Transl Med 2013;5:209ra151.ArticlePubMed 26
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That gives you 12 total weekly doses across three distinct dosage tiers, with four injections at each level
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