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Factors such as aging, stress, and environmental exposure can deplete your glutathione levels, leading to fatigue, dull skin, and a weakened immune system

The chosen conditions cover two common stress factors, reduced and maximal carbon catabolite repression, and two nitrogen sources (urea versus ammonium sulfate)

Human interleukin 1 alpha and interleukin 8 enzyme-linked immunosorbent assay (ELISA) kits were purchased from Biolegend (San Diego, CA, USA)

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GS then catalyzes the second step, consuming another molecule of ATP to add glycine to -Glu-Cys, ultimately forming GSH