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Future studies will reveal the applicability of our model, for example, to activate enzymatically inactive glutaredoxins, to alter the sensitivity and responsiveness of glutaredoxin-coupled fluorescent redox sensors, to further address the catalytic mechanism with GSSCys, HEDS and physiological substrates, or to design specific transition-state inhibitors

Intracellular concentrations of GSH are usually very high, ranging from 0.5 to 10 mM, whereas extracellular concentrations are found in the micromolar range 50

At acidic pH (below 5), deamidation occurs through direct hydrolysis and is generally slower

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AMPK has been called the master metabolic switch of the cell

This knowledge subsequently leads to successful and sustainable therapy