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5 L of each sample was analysed on a Acquity UPLC HSS-T3 column (50 2.1 mm, 1.8 m, Waters, MA, USA) by using gradient elution of 0.1% formic acid ( v / v ) in water as mobile phase A and 0.1% formic acid ( v / v ) in acetonitrile as mobile phase B, with a flow rate of 0.4 mL/min with the following gradient: 00.5 min (0% B), 0.8 min (15% B), 1.2 min (80% B), 1.4 min (80% B), 1.5 min (0% B), 2 min (0% B)

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Visible melanogenesis begins at 1421 days but continues deepening through week 8, with minimal erythemal dose increasing 24 at cycle completion

Anh-Tuan Truong, MD, FACS, FAACS, a triple board-certified cosmetic surgeon certified by the American Board of Surgery, the American Board of Cosmetic Surgery, and the American Board of Facial Cosmetic Surgery

Cells were washed, collected and resuspended in 0.3 ml of PBS

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