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doi:10.1023/A:1024034508136 Wei G, Wang D, Chen J (2008) Overproduction of Glutathione by L-cystein addition and a temperature-shift strategy
References: [1] Wu G, et al
The GPX3 of A
ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C
Storage conditions before purchase affect peptide stability
For glutathione injections specifically, the relevant standard is USP 797 the sterile compounding framework
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