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The primary antibodies used were anti- VEGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti- VEGFR1 (Cell Signaling Technology, Berkeley, CA, USA), anti- pVEGFR1 (Cell Signaling Technology, Berkeley, CA, USA), anti- AKT (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti- pAKT (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti- ERK (Cell Signaling Technology, Berkeley, CA, USA), anti- pERK (Cell SignalingT echnology, Berkeley, CA, USA), anti- p38 (Cell Signaling Technology, Berkeley, CA, USA), anti- p-p38 (Cell Signaling Technology, Berkeley, CA, USA), anti- eNOS (Proteintech, Chicago, IL, USA), anti- p-eNOS (Proteintech, Chicago, IL, USA), anti- CHOP (Cell Signaling Technology, Berkeley, CA, USA) and anti- -actin (Cell Signaling Technology, Berkeley, CA, USA)