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Obesity and overweight, which are common in patients with DM2, are a factors that favor the development/progression of CKD through both direct and indirect effects.69 Although the precise mechanisms the association between obesity and CKD are not totally defined it has been proposed that hypertension, glomerular hyperfiltration, hyperglycemia, lipid toxicity, inflammation or the role of adipose tissue and its adipokines are involved.70 Weight reduction by bariatric surgery, pharmacological treatment or dietary measures are associated with a nephroprotective effect,7173 so that weight reduction is a fundamental objective in the nephroprotection of patients with DKD

Additionally, when examining immobility per minute, a two-way repeated measures ANOVA showed a significant effect of treatment over time (F(1, 22) = 8.643, p = 0.0076), with semaglutide-treated mice spending significantly less time immobile in minutes 3, 4, and 5 compared to controls (p values of 0.0309, 0.0001, and 0.0131, respectively)

The GST protein is fused to 20 amino acid His-Tag at N-terminus

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Sugiharto S, Pratama AR, Yudiarti T, Wahyuni HI, Widiastuti E, Sartono TA