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Insulin-like growth factor 1 deficiency exacerbates hypertension-induced cerebral microhemorrhages in mice, mimicking the aging phenotype

Once this period has passed, the pen should be discarded

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Briefly, 24 hours after transfection, cells were washed with PBS, detached, and resuspended in NanoGlo Live Cell Reagent (Promega) with furimazine (1:40 dilution in HBSS) and seeded into white 96-well half-area plates