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Methylation SAM and SAH SAM-to-SAH ratio Methionine Homocysteine Minerals and Antioxidant Context Plasma zinc Serum copper Ceruloplasmin Glutathione-related markers when appropriate Organ Safety CBC and comprehensive metabolic panel Creatinine, eGFR and urinalysis AST, ALT, GGT and bilirubin Serum bicarbonate Heavy-metal, mold, gut or environmental testing should follow a credible exposure history rather than mood symptoms alone