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Let the alcohol evaporate for 30 seconds before proceeding

Secondary antibodies, Alexa 596 (#A-11012, Thermo Scientific, USA) and Alexa 488 (#A-21202, Thermo Scientific, USA) were used at 1:1000 dilution for double labeling for 30 min at RT

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10.1016/j.bbagen.2012.11.020 12 BrownC