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A vial punctured on day 1 of its 18-month shelf life and a vial punctured on day 500 both carry the same 28-day post-puncture window assuming the manufacturer-printed expiration has not yet arrived

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Step 2: Using the mixing syringe tighten the needle to the hub then pull hack the plunger to bring in 10 mL/cc of air

doi: 10.1124/mol.108.051516 74 FernandesN.NeroL.LyonsS.IvanovP.MittelmeierT.BolgerT.et al