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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

Ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry, two-dimensional liquid chromatography for resolving co-eluting impurities, and capillary electrophoresis for sequence verification are among the techniques that GLP-1 manufacturers have had to develop and validate at GMP scale to meet the analytical control requirements of regulatory submissions

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