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For the tumour allograft model, Gclc f/f Rosa26 creERT2 MMTV-PYMT mice were euthanized and autochthonous tumours were collected, cut into 8 mm 3 fragments and cryopreserved in liquid nitrogen

In addition, dichloroaceetate treatment alone caused ER stress to an extent that was nearly as robust as the bona fide ER stressor TM, as seen by upregulation of UPR target genes (Figure 7B) and splicing of Xbp1 (Figure 7C)

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