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Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)

Skin and Other Effects: Very rarely, severe skin rashes or abdominal pain have been reported

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Exogenous substances like N-acetylcysteine can raise the intracellular amounts of GSH [110]

( d ) NAO staining of mitochondrial mass in HT29 cells treated 5FU for 72 h, n = 3 biological replicates, with mean s.e.m

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