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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

Pellagra and alcoholism: A biochemical perspective

doi: 10.1038/s42255-020-00327-x 16 ZhaoXWangMWenZLuZCuiLFuCet al