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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)

Additionally, treatment with 10 nM E2, for 7 days, stimulated differentiation to the neuronal lineage, and inhibited differentiation to astrocytes (Zhang et al., 2019)

Its a gut peptide

Also, frost-free freezers should be avoided to store peptides, as temperatures can fluctuate widely during defrosting cycles.