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147 Calik, M
In chemotherapy-sensitive patients, Keap1 (Kelch-like ECH-associated protein 1) binds to NRF2 and mediates NRF2 ubiquitination via the Cul3-E3 ligase complex, promoting its degradation and maintaining NRF2 at low levels [32]
As GSH patrols the cellular environment and puts out oxidative free radical fires, it becomes oxidized and inactive, thus turning into GSSG
Copper (II) sulfate pentahydrate, iron (III) chloride hexahydrate, ascorbic acid, disodium ethylenediaminetetraacetate dihydrate (EDTA), 5,5-dithiobis-2-nitrobenzoicacid (DTNB), tris(hydroxymethyl)aminomethane (TE8), cysteine, -L-glutamyl-L-cysteine and glutathione were purchased from Sigma Aldrich (St Louis, USA)
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