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In the case of compound 2-bound structure, the N-terminal -helix of ECD penetrated to the TMD core and formed extensive contacts with residues in TM1-3, ECL1 and ECL2, resulting in an ECD-TMD interacting surface area of 1438.4 2 , similar to other small molecule agonists
31 The mutation of GIP Ile 12 to Ala caused a notable decrease in its insulinotropic effect
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