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Even if youre purifying the same protein over and over, it is probably still a good idea to clean and regenerate your column about every five purifications, or earlier if you notice that the binding capacity of your beads is getting worse
Lancet 1968;2:849-52
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With exception of p -cresol sulfate (a known protein degradation product in bacterial metabolism 37 ) and 4-hydroxphenylpyruvate, the top accumulating metabolites belonged to mono- and polyunsaturated fatty acids (Fig
6a), similar to in vivo levels
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