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When reconstituted with a sterile diluent, it maintains structural integrity for experimental testing

To investigate the effect of gene knockout on l-cysteine and GSH degradation, the engineered strains were cultured in the flasks as described above and the cells were collected by centrifugation (6000 rpm, 4 C for 5 min) after two washes with 50 mM phosphate buffer (pH7.0)