RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)
They are not intended for human consumption, diagnosis, treatment, or prevention of any disease
The dose of oral vitamin C ranges between 500 mg once to twice daily, with a maximum of 2000 mg daily
Always consult a qualified and registered medical practitioner before commencing any new therapy
Key Safety Insights inlcude: Hypoglycemia: IGF-1 enhances glucose uptake into muscles
Methylation of the DFNA5 gene is frequently detected in colorectal cancer