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In vitro glucose-stimulated insulin secretion (GSIS) Insulin secretion was measured using the Krebs Ringer Bicarbonate (KRB) buffer (119 mM NaCl, 5 mM KCl, 2.5 mM CaCl 2 , 1.2 mM KH2PO 4 , 1.2 mM MgCl 2 , 10 mM HEPES, 25 mM NaHCO 3 , and 0.2% BSA)
Care 7 (1), e000783
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Gastric surgery, such as weight loss procedures, can also lead to vitamin B12 deficiency by reducing the number of cells needed to secrete stomach acid and intrinsic factor
For less severe but concerning symptoms, contact NHS 111 for advice