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Each spine hosts one or at times several synapses coming in from other neighboring neurons
Full geometry optimizations, relaxed scans and transition structure searches were carried out with Gaussian 16 83 with the ONIOM 84 QM/MM method using the B3LYP hybrid functional 85 and 6-31 G(d) basis set with ultrafine integration grids for the QM part and the ff14SB 78 , gaff2 79 and TIP3P 86 force fields for the MM part (protein, substrate and water, respectively) with electrostatic embedding
The results can last from several weeks to a few months, and then it may be time for a follow-up appointment
PMID 1591472.
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