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After three washing, diluted streptavidin- Horseradish peroxidase (HRP) was added to each well and plate was incubated for 60 min at room temperature

10.1016/j.joca.2015.07.008 Osteoarthritis Cartil

The morphology of mitochondria was studied by transmission electron microscopy (TEM) using a Hitachi TEM system (HT7800, Tokyo, Japan)

We found a significant inverse correlation between systemic TNF and NO levels

We thus tested the activity of alkaline phosphatase PhoA in different E