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However, some plans may allow exceptions if strong documentation of medical necessity is provided

Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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It has a sulfhydryl (SH) group on the cysteinyl portion, which accounts for its strong electron-donating character

We can't stress this enough: for any research protocol that requires drawing from the same vial more than once, bacteriostatic water is the only appropriate choice

L., Murphy, J