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Cells were suspended in HBSS, placed into black, clear-bottomed plates, and FRET was measured before and after agonist addition in a Flexstation 3 plate reader at 37C using the following settings: ex = 440 nm, em = 485 and 535 nm

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In addition Arg 40* interacts with Tyr 32 (CDR1 VL) through a cation- effect