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Standard / Gradual Approach A representative reference vial is compounded at roughly methionine 25 mg/mL + inositol 50 mg/mL + choline 50 mg/mL (125 mg/mL combined MIC) with ~1000 mcg/mL B12 , though ratios such as 25/25/25 are equally common [1]
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Dried polar samples were resuspended in 100 L water and 2 L were injected into a ZIC-pHILIC 150 x 2.1 mm (5 m particle size) column (EMD Millipore)
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