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PMID: 9849822 Sigalos JT, et al

However, many mothers find that when they first start breastfeeding they experience soreness or pain in their nipples and sometimes their breasts

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These systemic effects are uncommon at standard therapeutic doses

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RNA isolation and quantitative RT-PCR Samples for gene expression analysis were frozen and ground in liquid nitrogen and then total RNA purification, reverse transcription, and qRT-PCR were completed using one-step kit (TransGen Biotech)