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Each reaction contained 0.4 g TMPK or 0.3 g CMPK was incubated at 25 C followed by using Tecan Spark 10 microplate reader to detect the change in NADH measurement at 340 nm absorption according to the manufacturers instructions

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The median weight loss across published trials is 4 to 6 pounds, and most of that occurs in the first 12 weeks with minimal further loss afterward