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Biochemistry 32 , 33683376 (1993)

What you do after matters just as much

Its a small peptide made up of three amino acids attached to a copper ion

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature