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Zhang L, Ludden CM, Cullen AJ, Tew KD, Branco de Barros AL, Townsend DM

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Excess adiposity, particularly visceral fat, is associated with metabolic dysfunction that may affect hormone regulation

Brains were collected and stored in 4% PFA for 24 h after which they were transferred to 20% sucrose for cryoprotection at 4 C