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a , Relative metabolite abundance of GSSG and GSH in whole cell lysate (WC) and isolated ER fractions from HEK293T cells treated with or without 1 mM BSO for 48 h

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Two key enzymes, acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3), are involved in the biosynthesis and remodeling of PEs, activation of PUFAs, and insertion of PUFAs into membrane PLs (Doll et al., 2017)

However, given that the ratio of GSH to GSSG in the cytosol is approximately 100:1, it seems unlikely that cytosolic GSH is limiting in this way

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