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Figure 1 The TME of osteosarcoma is characterized by a dense, mineralized extracellular matrix (ECM) and the infiltration of immunosuppressive cell populations that collectively orchestrate resistance to immunotherapy

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Moreover, Cyp2e1-/- mice showed resistance to the APAP high dose-associated hepatotoxicity, whereas human CYP2E1 transgenic mice were susceptible, suggesting that CYP2E1 is the major P450 enzyme participating in APAP activation ( via O-glucuronidation (50%70%) and O-sulfation (approximately 25%) pathways and forms the corresponding conjugates that can be easily excreted by the kidney