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Following three washing steps with PBS, cells were permeabilized with 0.1% Triton for 20 min and blocked with 2% BSA in PBS-Tween (0.5%) at room temperature for at least 1 h

Fresh solutions were prepared every 2 weeks, given at a dose of 0.1 ml (25 mg)/kg b.wt

En el citosol, la formacin de PSSG es transitoria, excepto durante el estrs oxidativo, pudiendo estar implicada en un mecanismo de transduccin de seal en respuesta a estrs que permita su adaptacin o determine su apoptosis

5 10 6 cells transduced with a scrambled shRNA or shACTL6A were inoculated subcutaneously into the hind-flanks of 6-week-old male BALB/ c-nu/nu mice

Low zinc may reduce metallothionein support and worsen the functional imbalance between zinc and copper

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