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Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 LR3 which had been pre-incubated in conditioned media for 2 or 7 days

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Of note, the dose of GLP-1(2836) used was selected on the basis of its dose response effect on post-IRI LVDP (Supplemental Figure 2)

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