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Similarly, there appeared to be a proliferation of DPCs, though this result was not entirely certain

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Normal dermal fibroblasts were seeded into a 96-well cell culture plate and have been grown to confluence in high glucose media, supplemented with 10% fetal calf serum for around 3 days

doi: 10.1007/BF02978833

This process apparently starts when the VEGF protein, which is considered to be secreted by oxygen-deprived cells during a hypoxic response, and may bind to VEGF receptors on the surface of endothelial cells

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