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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection
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Reading the structure of IGF-1 LR3 The full sequence, formula and mass for this listing are printed in the specification table further down this page rather than restated here
Supplementation (typically 500-2000 mg daily) can boost its effects
The route to get here differs DAC through sustained elevation, no-DAC through repeated pulsatile stimulation but the cumulative IGF-1 exposure is comparable with proper protocols
