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The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 and 100 M TCEP (Sigma-Aldrich) supplemented with EDTA-free protease inhibitor cocktail (TragetMol) by dounce homogenization

However, common adverse reactions such as gastrointestinal reactions and hypoglycemia often occur

Efficacy and Safety of Once-Weekly Semaglutide Versus Once-Daily Sitagliptin as an Add-on to Metformin, Thiazolidinediones, or Both, in Patients With Type 2 Diabetes (SUSTAIN 2): A 56-Week, Double-Blind, Phase 3a, Randomised Trial

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Younossi Liver International Communications .2026;[Epub] CrossRef Evolving B-mode ultrasound-based techniques for assessing metabolic dysfunction-associated steatotic liver disease: Now and beyond Walaa Abdelhamed, Mohamed Elbadry, Mohamed El-Kassas Liver Research .2026