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A 2012 review of all known studies determined that daily B12 losses in apparently healthy adults and elderly range from 1.4 to 5.1 g (Doets, 2012)

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It is a short peptide in which the copper atom is attached between the histidine and alanine residues of the alanine-histidine-lysine tripeptide

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The gauge number refers to needle thickness: the higher the gauge, the thinner the needle