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Afterwards, cells were lysed by three freeze and thaw cycles in liquid nitrogen and a 37 C waterbath, followed by addition of 900 L resuspension buffer (10 mM MgCl 2 , 10 mM ZnCl 2 , 1 M Tris-HCl, pH 8.0) and warming the samples to 28 C

PubMed laboratory research record [PMID: 22709675] PubMed record related to this compound for laboratory literature review